Ultrasound Method is First to Restore Dopaminergic Pathway in Brain at Early Stages of Parkinson's Disease

June 6, 2019

Columbia biomedical engineers use transcranial ultrasound and intravenously inject microbubbles to open a pathway through the blood-brain barrier for drugs to penetrate and trigger therapeutic effects—FDA has approved their device for clinical trials in Alzheimer’s patients

Credit: Maria Eleni Karakatsani/Columbia Engineering

Upregulation of the dopaminergic pathway following focused ultrasound-facilitated drug delivery The dopaminergic pathway can be downregulated similar to Parkinson’s disease in toxin-based mouse models. Application of focused ultrasound results in increased blood-brain barrier permeability allowing the diffusion of pharmacological agents in the brain. Depending on the number of administrations and the delivery vehicle, the deliverable compounds can have beneficial effects of varying degree.

Credit: Antonios Pouliopoulos /Columbia Engineering 

Portable ultrasound system for targeted and non-invasive blood-brain barrier opening in humans. The system was recently cleared by the FDA for ultrasound treatment of 6 US Alzheimer's disease patients.

Ultrasound Technique First to Restore Dopaminergic Pathway in Brain at Early Stages of Parkinson's Disease 

Columbia biomedical engineers use transcranial ultrasound and intravenously inject microbubbles to open a pathway through the blood-brain barrier for drugs to penetrate and trigger therapeutic effects—FDA has approved their device for clinical trials in Alzheimer’s patients 

Newswise — New York, NY—June 6, 2019—While there are several thousand drugs available to treat a wide range of brain diseases, from depression to schizophrenia, they cannot penetrate the blood-brain barrier (BBB) into the brain. The BBB, which protects the brain from pathogens that may be present in blood, also prevents most drugs from gaining access to the brain  functional tissue, the parenchyma, a well-known challenge to the treatment of all brain diseases including neurodegenerative disorders like Parkinson's disease and Alzheimer’s. 

A team led by Elisa Konofagou, Robert and Margaret Hariri Professor of Biomedical Engineering and Professor of Radiology (Physics) at Columbia Engineering, has been developing a novel technique that could open up new ways to facilitate targeted drug delivery into the brain and enable drugs to treat brain diseases more focally. The researchers used transcranial, focused ultrasound and intravenously injected microbubbles into the BBB to make a localized and transient opening that allows drugs to cross through the BBB reversibly and non-invasively. Focusing on Parkinson’s disease, in collaboration with Serge Przedborski’s group in the department of neurology at Columbia University Irving Medical Center, they discovered that protein delivery and gene delivery across the BBB can partly restore the dopaminergic pathways, the neurons in the brain that are affected in early Parkinson's disease. 

“We found both behavioral and anatomical neuronal improvements in the brain,” says Konofagou, who led the study, published online April 4 by the Journal of Controlled Release, and in print June 10. “This is the first time that anyone has been able to restore a dopaminergic pathway with available drugs at the early stages of Parkinson's disease. We were able to curb the rapid progression of neurodegeneration while improving the neuronal function. We expect our study will open new therapeutic avenues for the early treatment of central nervous system diseases.” 

The team targeted the brain regions involved in early stage Parkinson's and Alzheimer's disease, such as the putamen and hippocampus. The tool they developed for the study is a device that uses a neuronavigation system to direct the treatment in real-time. The U.S. FDA has just assigned the team an Investigational Device Exemption (IDE) to use the device in clinical trials to test its safety in Alzheimer's patients. 

“Neurosurgeons use such systems all the time to guide them for neurosurgery,” says Antonios Pouliopoulos, associate research scientist in Konofagou’s lab who worked on the development of the clinical neuronavigation system. “Our group just replaced the surgical instrument with an ultrasound transducer to perform our non-invasive procedure.” 

Konofagou’s Ultrasound Elasticity and Imaging Laboratory is the only academic lab in the U.S. to receive FDA approval for ultrasound-assisted, blood-brain-barrier opening. Other groups doing similar research either use nanoparticles to facilitate drug delivery or require MRI to guide the procedure. Konofagou’s approach is MRI-independent and does not require any nanoparticles. 

Her device is a single-element transducer that is much smaller, faster, and less expensive than current helmet-shaped, 1024-element transducer systems that use MRI guidance. Because Konofagou’s system is portable, doctors will be able to treat patients anywhere in a hospital and, in the future, even at a patient’s home. Treatment can be completed in less than 30 minutes, compared to three to four hours for MRI-guided therapy, and monitored in real-time, a unique feature of the new device.  The cost is 10 times less than the MRI-guided helmet. The first trial with the device will be with Alzheimer's patients, after which Konofagou plans to work with Parkinson's patients. 

Konafagou recently won a four-year $2.5M NIH grant to use a similar device for deep brain stimulation aiming to unveil the mechanism by which ultrasound excites neurons and to monitor the unveiled mechanism in human subjects. In addition, she will be honored with the 2019 Engineering in Medicine and Biology Society’s Technical Achievement Award in Berlin this July for her “outstanding and pioneering contributions in the field of ultrasound imaging and therapy, and their application and clinical translation to the diagnosis of cardiovascular diseases, tumor diagnosis and treatment as well as brain drug delivery and stimulation.” 

“We all have loved ones with neurodegenerative disorders,” Konofagou adds. “My grandmother has been suffering from dementia for more than five years, so I know first-hand how essential it would be to have a simple device that can be wheeled into the patient's home and offer a higher quality of life, especially for our rapidly aging population. And there are so many deadly diseases like brain tumors that affect people of all ages, with no cure yet in sight. That’s why we want to bring our research so rapidly to the clinic.” 

About the Study 

The study is titled “Amelioration of the nigrostriatal pathway facilitated by ultrasound-mediated neurotrophic delivery in early Parkinson's disease.” 

Authors—all from Columbia University—are: Maria Eleni Karakatsani a,; Shutao Wang a; Gesthimani Samiotaki a; Tara Kugelman a,;Oluyemi O. Olumolade a; Camilo Acosta a; Tao Sun a; Yang Han a; Hermes A.S. Kamimura a; Vernice Jackson-Lewis c, e, f; Serge Przedborski c, d, e, f; Elisa Konofagou a, b, f.a Department of Biomedical Engineeringb Department of Radiologyc Department of Pathology & Cell Biologyd Department of Neurologye Center for Motor Neuron Biology and Diseasef Columbia Translational Neuroscience Initiative 

The study was supported by the National Institutes of Health, (AG038961, EB009041, NS099862, NS072428, and NS107442), the Focused Ultrasound Foundation, the Kavli Institute, and the Kinetics Foundation. 

The authors declare no financial or other conflicts of interest. 

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LINKS:

Paper: https://www.sciencedirect.com/science/article/pii/S0168365919301907?via%3Dihub
DOI: 10.1016/j.jconrel.2019.03.030
http://engineering.columbia.edu/
https://engineering.columbia.edu/faculty/elisa-konofagou
http://orion.bme.columbia.edu/ueil/
https://www.cuimc.columbia.edu/
https://www.journals.elsevier.com/journal-of-controlled-release
https://bme.columbia.edu/

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Columbia Engineering

Columbia Engineering, based in New York City, is one of the top engineering schools in the U.S. and one of the oldest in the nation. Also known as The Fu Foundation School of Engineering and Applied Science, the School expands knowledge and advances technology through the pioneering research of its more than 220 faculty, while educating undergraduate and graduate students in a collaborative environment to become leaders informed by a firm foundation in engineering. The School’s faculty are at the center of the University’s cross-disciplinary research, contributing to the Data Science Institute, Earth Institute, Zuckerman Mind Brain Behavior Institute, Precision Medicine Initiative, and the Columbia Nano Initiative. Guided by its strategic vision, “Columbia Engineering for Humanity,” the School aims to translate ideas into innovations that foster a sustainable, healthy, secure, connected, and creative humanity.

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